Lasers in Laboratory Studies:
The Need for Special Systems
Laboratory studies of low level laser therapy (LLLT) need special laser systems to deliver consistent laser irradiation parameters. A typical uncorrected diode laser beam is neither round nor homogeneous. If light is delivered by fiber optics, the beam will be round but not homogeneous. With these non-homogeneous beams, cells irradiated in vitro and in vivo are exposed to different irradiances.
Some cells may not be exposed to the required intensity or energy threshold for stimulation, while others may be irradiated to the point of inhibition. If we believe that all cells in vitro should be exposed to the same radiant intensity, then a homogeneous "flattop" distribution is necessary.
Other variations in irradiation parameters can occur when using laser diodes. Laser diodes heat up during use which causes the wavelength to shift 0.4nm for every 1 degree C rise in temperature. Furthermore, during operation power output drops significantly in many laser systems. These changes in wavelength and power output mean that during the course of an experiment where multiple samples are irradiated, samples would be subjected to different wavelengths, irradiances and energy densities.
The benefits of this new device include: tune-able output power (potentially less than 1mW up to several watts depending on your requirements), round, even beam power distribution, and stable wavelength and power outputs.
Wavelength and power output stability are achieved by constant real time photodiode feedback and heat stability is managed by thermoelectric Peltier cooling. Round homogeneous beam distribution is achieved with fibre optics, a beam diffuser and a variety of apertures to match required culture plate well size or area of animal model to be irradiated. Both in vivo and in vitro dose ranging studies will benefit from this attention to accuracy.
In addition to well controlled beam parameters the BioTHOR system has a countdown timer and a range of pulse options with variable duty cycle. We can support and advise you on your study design and are willing to supervise / collaborate on the writing of papers.
Prices are typically $15,000 but please contact us to discuss your specific requirements.